电离辐射通过转化酪氨酸-β-介导的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘录 ：论述电离辐射是否是可通过转化生长因子-β（TGF-β）-介导的上皮-间质转换 (EMT)来促进肿瘤细胞内的洪水泛滥迁到。用到量2Gy(60)Coγ线或紫外光源自人类人体器官的6种肿瘤细胞内，历史纪录与EMT相关的巨大变化，这包括分别运用显微镜关键技术，蛋白印迹方法，免疫荧光关键技术，划痕试验和Transwell小室试验来观察并监测细胞内组织形态，EMT标上，洪水泛滥迁到技能等。采用复合物排免疫吸附法监测这些肿瘤细胞内中TGF-β蛋白素质，运用特别依赖性剂SB431542来评估TGF-β瞬时通路在电离辐射EMT中的作用。经过量为2Gy紫外光的肿瘤细胞内中存在间叶细胞内的理解，与假紫外光组相比其上皮标上缩减，间叶细胞内标上增加，同时其洪水泛滥集中于技能增强，TGF-β蛋白素质也提高。进一步发现由A549电离辐射诱导的EMT可通过对TGF-β瞬时依赖性发生逆转。这些结果表明TGF-β介导的EMT在电离辐射诱导增强肿瘤细胞内洪水泛滥集中于技能中起着巨大作用。

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